iTOL-100

Potentially Transformational Regenerative Biotin-PEG Microgel Platform

What is Regenerative Medicine?

A class of medicines that aim to grow, repair or replace damaged or diseased cells, tissues or organs. Regenerative medicine goes beyond treating diseases or managing symptoms and seeks to develop cures. However, a significant shortcoming of cell therapy is the body’s immune system working to seek out and kill foreign cells, tissues, organoids and organs implanted into the body, requiring the need for life-long immunosuppression.

 

iTOL-100: Our SA-FasL Microgel Immune Tolerance Platform

Through a data-driven approach, we are working to enable regenerative medicine without the need for life-long immunosuppression. Our platform technology, iTOL-100, leverages the naturally occurring protein, Fas Ligand (FasL) which is present in all mammals and functions as a natural way for the body to establish local immune tolerance.

iTOL-100 is a biotin-PEG microgel fused with a chimeric fusion protein of streptavidin-FasL in a synthetic biomaterial (SA-FasL microgel), which is synthesized through a straightforward process. Ordinarily, activated T-cells will reject foreign tissue.  Current implantation of cells or organs require life-long immunosuppression to prevent organ rejection.  However, when we then mix iTOL-100 with the target cell therapy and implant it into the body, the FasL binds to the Fas receptor (FasR) on activated T-cells, preventing rejection through a process called apoptosis (or programmed cell death). In parallel, T-regulatory cells are generated creating localized immune privilege and protecting the implanted cell therapy.

 

Potential Key Technology Benefits 

No long-term immunosuppression
Potential to eliminate chronic life-long antiviral, antifungal and antibacterial drugs
Durable engraftment without rejection
Off the shelf
Modular
Applicable to multiple programs

In pre-clinical studies, our iTOL-100 platform has been shown to establish durable, localized immune tolerance, allowing the implanted tissue, organoid or cell therapy to function as a replacement for damaged native cells.

Mechanism
of Action

 

 Immobilized and
co-localized SA-FasL Microgel

Straightforward Procedure

Our proprietary technology has broad applicability and can be applied to both allogenic and stem cell-derived organoids across a number of diseases.

A potential cure for
Type 1 Diabetes

Liver failure

Regenerative protein and
cell therapy for diseases
with high unmet need